Hi all,
I am transducting primary mouse splenocytes, and was wondering if there is another method to determine the quality of the (in HEK293T) produced lentivirus? As when doing a titration in HEK or 3T3 cells, I always get very high (95+ %) efficacy, however back in the primary cells I am down to 10-20%, depending on the batch...
Therefore I am wondering if there is another way to assess how good or bad a batch is? Maybe PCR?
Thanks in advance :)
Thanks Serena,
So far I just transduced with different amounts of aliquot (of conc. lentivirus). My problem is rather that it is hard to estimate from the HEK transduction to the real efficacy in the splenocytes.
The lentiviral plasmids contain gene of interest T2A mCherry, and I assessed the efficacy by measuring mCHerry after 3 days using a FACS machine.
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