How can I extract EP(epithelial cells) from Breast tumor samples ?

09 October 2014 9,764 4 View

Can any one help me to estimate the effect of CO2 on MCF7 cell line culture?

I want to know if CO2 affects the growth of MCF7 cells or not.

09 October 2014 6,402 3 View

Can anyone help me in the choose another breast cancer stem cell markers besides CD24, CD44, ALDH1 markers?

I am already work on these markers in the isolation process of stem cells of breast cancer, and I need other markers to make isolation process perfect.

08 September 2014 9,470 2 View

Do you have a protocol for paraffin blocking of a cell line (MCF7)?

A detailed protocol that explain the method of blocking MCF7.

08 September 2014 6,025 2 View

How to do processing of the normal tissue indetail?

Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists.

08 September 2014 8,799 0 View

How to establish a breast cancer primary cell line?

Our group is working on breast cancer tissue doing cell culture, preparing for a future stem cell isolation based on cell markers. We could see too many cells floating in the media on the day...

11 December 2013 8,780 24 View

Is there a specific protocol in IHC technique for a normal breast tissue that differs from breast cancer tissue?

I have a problem in IHC technique for normal breast tissue (control) specific in antigen retrieval step where tissue didn't remain in slide. This problem appears only in normal breast tissue slide .

03 April 2013 9,751 6 View

what's the time required for fixing solid tumor in 10% formaldehyde for IHC buffer embedded tissue?

The type of the tumor that I am working on is breast cancer, it's size moderate, and it is from solid type. Is it right that the more times it is in fixed the better the result? If this is right,...

11 December 2012 8,943 18 View

Which stage is the safest and the best for preserving breast cancer samples 10% formaldehyde or 70% ethyl alcohol for a long time?

Is there anything better ?

11 December 2012 4,320 2 View

Is there are any method to get rid of calcification in breast tumor ?

The sample that I have contains a large amount of calcium which makes it very hard. It may become problem in sectioning of buffering embedded tissue .

11 December 2012 4,117 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View