We analyse the mammosphere-forming ability of cells derived from clinical samples. Prior to their use in the mammosphere assay, epithelial cells (EP) first need to be extracted from such...
09 October 2014 9,841 4 View
I want to know if CO2 affects the growth of MCF7 cells or not.
09 October 2014 6,484 3 View
I am already work on these markers in the isolation process of stem cells of breast cancer, and I need other markers to make isolation process perfect.
08 September 2014 9,589 2 View
A detailed protocol that explain the method of blocking MCF7.
08 September 2014 6,097 2 View
Our group is working on breast cancer tissue doing cell culture, preparing for a future stem cell isolation based on cell markers. We could see too many cells floating in the media on the day...
11 December 2013 8,863 24 View
During IHC protocol i will make blocking for alkaline phosphatase and i need mounting for this type of slides
05 June 2013 7,298 2 View
I have a problem in IHC technique for normal breast tissue (control) specific in antigen retrieval step where tissue didn't remain in slide. This problem appears only in normal breast tissue slide .
03 April 2013 9,836 6 View
Is there anything better ?
11 December 2012 4,414 2 View
The type of the tumor that I am working on is breast cancer, it's size moderate, and it is from solid type. Is it right that the more times it is in fixed the better the result? If this is right,...
11 December 2012 9,038 18 View
The sample that I have contains a large amount of calcium which makes it very hard. It may become problem in sectioning of buffering embedded tissue .
11 December 2012 4,186 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...
30 July 2024 7,062 1 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
