Do you have a protocol for paraffin blocking of a cell line (MCF7)?

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How can I extract EP(epithelial cells) from Breast tumor samples ?

We analyse the mammosphere-forming ability of cells derived from clinical samples. Prior to their use in the mammosphere assay, epithelial cells (EP) first need to be extracted from such...

09 October 2014 9,841 4 View

Can any one help me to estimate the effect of CO2 on MCF7 cell line culture?

I want to know if CO2 affects the growth of MCF7 cells or not.

09 October 2014 6,484 3 View

Can anyone help me in the choose another breast cancer stem cell markers besides CD24, CD44, ALDH1 markers?

I am already work on these markers in the isolation process of stem cells of breast cancer, and I need other markers to make isolation process perfect.

08 September 2014 9,589 2 View

How to do processing of the normal tissue indetail?

Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists.

08 September 2014 8,883 0 View

How to establish a breast cancer primary cell line?

Our group is working on breast cancer tissue doing cell culture, preparing for a future stem cell isolation based on cell markers. We could see too many cells floating in the media on the day...

11 December 2013 8,863 24 View

which mountain can i use for hydrated slides ( alkaline phosphatase ) in IHC technique?

During IHC protocol i will make blocking for alkaline phosphatase and i need mounting for this type of slides

05 June 2013 7,298 2 View

Is there a specific protocol in IHC technique for a normal breast tissue that differs from breast cancer tissue?

I have a problem in IHC technique for normal breast tissue (control) specific in antigen retrieval step where tissue didn't remain in slide. This problem appears only in normal breast tissue slide .

03 April 2013 9,836 6 View

Which stage is the safest and the best for preserving breast cancer samples 10% formaldehyde or 70% ethyl alcohol for a long time?

Is there anything better ?

11 December 2012 4,414 2 View

what's the time required for fixing solid tumor in 10% formaldehyde for IHC buffer embedded tissue?

The type of the tumor that I am working on is breast cancer, it's size moderate, and it is from solid type. Is it right that the more times it is in fixed the better the result? If this is right,...

11 December 2012 9,038 18 View

Is there are any method to get rid of calcification in breast tumor ?

The sample that I have contains a large amount of calcium which makes it very hard. It may become problem in sectioning of buffering embedded tissue .

11 December 2012 4,186 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View