Shrouk El khamisy

11 Questions 12 Answers 0 Followers

Questions related from Shrouk El khamisy

Can any one help me to estimate the effect of CO2 on MCF7 cell line culture?

I want to know if CO2 affects the growth of MCF7 cells or not.

10 October 2014 6,457 3 View

How can I extract EP(epithelial cells) from Breast tumor samples ?

We analyse the mammosphere-forming ability of cells derived from clinical samples. Prior to their use in the mammosphere assay, epithelial cells (EP) first need to be extracted from such...

10 October 2014 9,814 4 View

How to do processing of the normal tissue indetail?

Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists.

09 September 2014 8,854 0 View

Do you have a protocol for paraffin blocking of a cell line (MCF7)?

A detailed protocol that explain the method of blocking MCF7.

09 September 2014 6,075 2 View

Can anyone help me in the choose another breast cancer stem cell markers besides CD24, CD44, ALDH1 markers?

I am already work on these markers in the isolation process of stem cells of breast cancer, and I need other markers to make isolation process perfect.

09 September 2014 9,554 2 View

How to establish a breast cancer primary cell line?

Our group is working on breast cancer tissue doing cell culture, preparing for a future stem cell isolation based on cell markers. We could see too many cells floating in the media on the day...

12 December 2013 8,831 24 View

which mountain can i use for hydrated slides ( alkaline phosphatase ) in IHC technique?

During IHC protocol i will make blocking for alkaline phosphatase and i need mounting for this type of slides

06 June 2013 7,258 2 View

Is there a specific protocol in IHC technique for a normal breast tissue that differs from breast cancer tissue?

I have a problem in IHC technique for normal breast tissue (control) specific in antigen retrieval step where tissue didn't remain in slide. This problem appears only in normal breast tissue slide .

04 April 2013 9,810 6 View

Which stage is the safest and the best for preserving breast cancer samples 10% formaldehyde or 70% ethyl alcohol for a long time?

Is there anything better ?

12 December 2012 4,369 2 View

what's the time required for fixing solid tumor in 10% formaldehyde for IHC buffer embedded tissue?

The type of the tumor that I am working on is breast cancer, it's size moderate, and it is from solid type. Is it right that the more times it is in fixed the better the result? If this is right,...

12 December 2012 8,997 18 View

Is there are any method to get rid of calcification in breast tumor ?

The sample that I have contains a large amount of calcium which makes it very hard. It may become problem in sectioning of buffering embedded tissue .

12 December 2012 4,159 2 View