I need to use LB media at different pH (6.5/7/8) and I found that MOPS buffer has the right pKa that cover my range:
MOPS pKa at 25°: 7.14
MOPS pH range: 6.5 – 7.9
However, I don't know at which concentration should I use it.
Can anyone help me?
Reference: F. C. Neidhardt, P. L. Bloch, and D. F. Smith. 1974. Culture medium for enterobacteria. J Bacteriol 119(3): 736-747
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