Which MOPS concentration I should use to buffer my LB media?

Andrea Carobbi @Andrea-Carobbi

03 November 2021 1 7K Report

I need to use LB media at different pH (6.5/7/8) and I found that MOPS buffer has the right pKa that cover my range:

MOPS pKa at 25°: 7.14

MOPS pH range: 6.5 – 7.9

However, I don't know at which concentration should I use it.

Can anyone help me?

Cao Wenyan

Reference: F. C. Neidhardt, P. L. Bloch, and D. F. Smith. 1974. Culture medium for enterobacteria. J Bacteriol 119(3): 736-747

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