Andrea Carobbi

7 Questions 6 Answers 0 Followers

Questions related from Andrea Carobbi

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

06 August 2024 1,523 4 View

Which MOPS concentration I should use to buffer my LB media?

I need to use LB media at different pH (6.5/7/8) and I found that MOPS buffer has the right pKa that cover my range: MOPS pKa at 25°: 7.14 MOPS pH range: 6.5 – 7.9 However, I don't know at which...

03 November 2021 7,131 1 View

How can I remove the biofilm from my Bacillus liquid culture?

I'm working with the Bacillus subtilis wild type strain NCIB3610. I use to grow it on LB liquid media, 37 Celsius degree (culture tubes) overnight before performing my experiments. After the...

04 October 2021 1,042 6 View

What are the toxic activities affecting the bacterial periplasm?

Hi all, I'm aiming to study a bacterial purified protein known to be toxic in the periplasm. I haven't been able to predict the antibacterial activity of my protein by computational analysis and...

19 September 2021 1,904 1 View

Is there a limit of the plasmid size that can be transferred by triparental mating?

Hello colleagues, I'm trying to transfer the plasmid pKEB31:GOI carried by E. coli DH5alfa on Pantoea agglomerans using E. coli pRK2073 as helper. I did this triparental mating lots of time with...

17 October 2019 6,881 3 View

Why my mutant lose the vector so fast?

I create a mutant deleting a GOI and I complemented it using a vector carrying the removed GOI. I checked my strains by PCR using two pairs of primer, a pair for the removed gene and a second pair...

02 September 2019 624 1 View

Why I got DNA amplification in NTC and -RT samples (qPCR)?

Hi everybody, I carried out a qPCR in order to quantify expression level of two genes under different conditions. I used three pairs of primers, one for the endogenous control and two for the...

14 August 2019 3,752 5 View