29 May 2024 0 4K Report

I have a peculiar problem - in FBS or albumin containing media my cells (preadipocytes) are very easy to image (and therefore quantify using a combination of ilastik and CellProfiler).

However, in animal component/serum free media their boundaries on our phase contrast microscope become very faint, pretty much invisible. So we're unable to quantify the cell confluency in our well plates/cultures.

Why are cells in serum free media harder to image? And are there ways to address this (e.g., differential interference contrast microscopy)?

More John Yn's questions See All
Similar questions and discussions