Bfgf was aliquoted in media and transported at 22 degee Celsius. How long it is stable
Hello Aishwarya Lakshminarayanan
The article attached below will help.
Article Stabilisation of Recombinant Human Basic Fibroblast Growth F...
The study consisted of assessing the stability of FGF-2 (bFGF) at various temperatures, such as 4 °C, 25 °C and 37 °C for up to 48 hours, and their residual FGF-2 content was analysed by ELISA which was expressed as a percent of the baseline FGF-2 content obtained at t = 0. The FGF-2 samples were deemed sufficiently stable if their FGF-2 content did not fall by greater than 10%.
Best.
The stability of aliquoted basic fibroblast growth factor (bFGF) at 22 degrees Celsius (room temperature) can vary depending on several factors, including the formulation, purity, and specific storage conditions. Here are some general considerations:
Factors Affecting Stability:
General Guidance:
- Short-term Stability: Aliquoted bFGF stored at 22 degrees Celsius is generally stable for a few days to a couple of weeks, depending on the specific conditions mentioned above.
- Long-term Stability: For longer-term storage, it is recommended to store aliquots of bFGF at -20 degrees Celsius or ideally at -80 degrees Celsius to maintain stability over months to years.
Recommendations:
- Regular Monitoring: Periodically check the stability of aliquoted bFGF by performing bioactivity assays or testing protein integrity to ensure it remains suitable for intended applications.
- Aliquot Size: Consider aliquoting into smaller volumes to minimize freeze-thaw cycles and maintain stability during use.
Impression-
While aliquoted bFGF can be stored at 22 degrees Celsius for short periods, stability may vary. For longer-term storage and to ensure optimal stability and activity, storing at colder temperatures (-20°C or -80°C) is recommended. Regular monitoring and proper handling are essential to maintain the quality and efficacy of bFGF aliquots.