Should there be a correlation between band size on gel and SYBR green signal in the real time PCR run?

19 January 2016 4,703 3 View

Is there logic behind using Annexin V FACS with another antibody?

02 December 2015 605 3 View

High miniprep yield followed by extremely low Ct values?

I have been using Clone jet PCR cloning kit with a pJET1.2 blunt as a vector. The insert is from a clean PCR product that went through a blunting reaction (supplied with the cloning kit). The E....

19 October 2015 8,587 6 View

How do I use two way ANOVA on real time PCR results?

09 March 2015 8,424 6 View

I see primer-dimers only in my qRT-PCR negative controls. Does it mean everything is OK?

Hello, I have just started with some qRT-PCR for my reasearch and Im doing a calibration of my reactions. With all the primers I checked(5 genes) I get an amplification of my NTCs and NRTs, in a...

06 January 2015 5,961 8 View

How do I calculate standard errors for normalized log2 converted gene expression data?

I ran an experiment with a ref gene (3 biological repeats x 3 technical repeats) and normalized gene expression of my target genes to the ref gene. I decided to present the data as log2 fold...

05 January 2015 6,166 6 View

Has anyone had any experience with storing cell culture slides for a TUNEL assay?

22 December 2014 3,895 2 View

Can anyone recommend a process for preparing and storing slides for a TUNEL assay?

29 September 2014 8,964 2 View

Does anyone have a specific recommendation for a DNA pol or mix fit for PCR ?

I'm using bisulfite treated DNA as template for sequencing purposes.

07 September 2014 8,426 6 View

Any problems using the same primer set twice?

Theoretically, is there a problem with running a PCR and then running the same system again, with the same primers, with the product of the first reaction to further amplify the product? The...

20 August 2014 3,814 8 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View