I transformed BL21 and DH5a with a NAPE-AT plasmid. NAPE expression was similar in both strains. Now, I'm planning an animal study and need to use the strain that has better colonization or the one with lower negative effects on the animal.
Dear
According to me I recommend BL21 because it hasare used for lack many proteases and could have least negative effect on animal. Check some links.
Cordilay
https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency
https://www.neb.com/products/c2527-bl21de3-competent-e-coli
Thank you so much
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