Which lab strain of E. coli has better colonization, BL21 or DH5alpha?

More Noura S. Dosoky's questions See All

How can principles of sustainable agriculture be integrated into the workplace environment??

To achieve sustainability in the work environment, how will we use agriculture in it?

04 August 2024 5,049 4 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View

How can I use imagej to measure intensity of Bromophenol stain for protein demonestration?

I have slides stained by mercuric Bromophenol stain, and I want to use imageJ to measure its intensity? How can I do that. Besides what's the difference between H&E and H&E2 in imageJ?

30 November 2023 5,833 3 View

Does anybody know Matlab codes for optimal placement and sizing of electric vehicles charging stations?

i need to know Matlab codes for optimal placement and sizing of electric vehicles charging in distribution power systems using different techniques.

12 September 2023 6,674 3 View

Hello researchers, anyone here who works on the determination of self-absorption coefficients using the LIBS spectrosccopy ?

I need some help.

18 August 2023 8,618 2 View

What is the qos of layer 2 and 3 switches ?

Hi there i have research to work on . in that title i mentioned it. Can anyone recommend me or explain or any advice to do that research. My knowledge in network are bad so i dont get the...

16 August 2023 2,848 3 View

How do I cite an aphorism in research paper?

I want to add an aphorism in my research paper, but I don`t Know if this needs citation also, how to add it.

16 February 2023 881 3 View

Hello guys, how do I create a library on the Internet ?

I mean what are the programming languages used in building libraries on the Internet I am still a beginner and I would like to build a library that contains various books and I want your advice...

26 October 2022 1,802 2 View

Is there anyone doing research on t he extent of enhancing English language competence and performance through translation ?

This is my research

15 March 2022 304 4 View

Why Taguchi method is mainly used with Grey relational analysis than other designs of experiment ?

Nearly in all papers of Grey relational analysis, Taguchi method is used to complement it than other types of experimental designs such as central composite design. Why?

23 September 2021 1,917 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Marius K. Somda

Dear

According to me I recommend BL21 because it hasare used for lack many proteases and could have least negative effect on animal. Check some links.

Cordilay

https://www.neb.com/products/c2988-neb-5-alpha-competent-e-coli-subcloning-efficiency

https://www.neb.com/products/c2527-bl21de3-competent-e-coli

Noura S. Dosoky

Thank you so much