Is it possible to use 100% Ethanol as mobile phase for C18 or any reversed phase columns?

I want to separate 2 compounds on HPLC, and I have made the preliminary studies on TLC using reversed phase plates and 100% ethanol as mobile phase which achieved complete separation. Now I want...

13 August 2020 2,229 3 View

Is there any different in phage precipitation with PEG 8000 and PEG 6000 ???

Can the moleculer weight of PEG (8000 and 6000) cause a noticeable effect in phage precipitation???

22 December 2018 531 3 View

Is there a way to extract vanilla oil?

I tried distilling vanilla beans (hydrodistillation) but did not get any oil after 4 hours. However, the hydrosol had the same odor of vanilla. Is there a way to get the vanilla oil? Thank you!

07 October 2018 7,400 3 View

I need the Diabetes Quality of Life Questionnaire for my project, where can I find it?

The main objective of my project is to assess the psychosocial aspect of the diabetic patients especially type 1 (adolescent) . Do you have any suggestion on how can I assess this issue?

22 June 2018 1,167 3 View

Why sandalwood oil is prepared from the heartwood and not the sapwood?

Sandalwood oil is extracted from the heartwood. Is there a reason for or benefit from producing the oil in the heartwood?

13 February 2018 9,172 1 View

What are the factors required to be considered for wsn using on-demand query based apporach ??

20 April 2016 8,149 5 View

How can we make a hybrid dynamic system for fisher KKP equation?

discrete time piecewise smooth system continuous time piecewise system

15 November 2015 4,562 2 View

What is the best way to fractionate a methanolic extract?

Is fractionation by simply shaking with solvents of increasing polarity successively sufficient or is it needed to always have 2 immiscible solvents?

31 October 2013 8,863 32 View

Top Ten Learning Algorithm?

01 January 1970 4,217 33 View

Why it is required to have plasmid to have polymorphic immunity in a bacterial population?

Except CRISPR, what can the plasmid carry out as an immunity against phage??

01 January 1970 3,044 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

How to increase bacterial concentration of DNA from Laser capture microdissection samples?

I used laser capture microdissection, selected specific bacteria presented in ethanol-fixed slides. And DNA was extracted by Qiamp mini kit. The problem right now is we have pretty low...

09 February 2021 3,908 2 View