Is it possible to use 100% Ethanol as mobile phase for C18 or any reversed phase columns?

I want to separate 2 compounds on HPLC, and I have made the preliminary studies on TLC using reversed phase plates and 100% ethanol as mobile phase which achieved complete separation. Now I want...

13 August 2020 2,229 3 View

Is there any different in phage precipitation with PEG 8000 and PEG 6000 ???

Can the moleculer weight of PEG (8000 and 6000) cause a noticeable effect in phage precipitation???

22 December 2018 531 3 View

Is there a way to extract vanilla oil?

I tried distilling vanilla beans (hydrodistillation) but did not get any oil after 4 hours. However, the hydrosol had the same odor of vanilla. Is there a way to get the vanilla oil? Thank you!

07 October 2018 7,400 3 View

I need the Diabetes Quality of Life Questionnaire for my project, where can I find it?

The main objective of my project is to assess the psychosocial aspect of the diabetic patients especially type 1 (adolescent) . Do you have any suggestion on how can I assess this issue?

22 June 2018 1,167 3 View

Why sandalwood oil is prepared from the heartwood and not the sapwood?

Sandalwood oil is extracted from the heartwood. Is there a reason for or benefit from producing the oil in the heartwood?

13 February 2018 9,172 1 View

Did anyone had an experience with [ LCO 1490 & HCO2198 ] primers for PCR? are these primers effective in the genetic barcoding of Earthworms?

I would like to find out more about these primers, How can i get them, from where, and finally how much it would cost ?

18 May 2017 7,940 5 View

What are the factors required to be considered for wsn using on-demand query based apporach ??

20 April 2016 8,149 5 View

How can we make a hybrid dynamic system for fisher KKP equation?

discrete time piecewise smooth system continuous time piecewise system

15 November 2015 4,562 2 View

What is the best way to fractionate a methanolic extract?

Is fractionation by simply shaking with solvents of increasing polarity successively sufficient or is it needed to always have 2 immiscible solvents?

31 October 2013 8,863 32 View

Top Ten Learning Algorithm?

01 January 1970 4,217 33 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View