6 Questions 14 Answers 0 Followers
Questions related from Noura S. Dosoky
I tried distilling vanilla beans (hydrodistillation) but did not get any oil after 4 hours. However, the hydrosol had the same odor of vanilla. Is there a way to get the vanilla oil? Thank you!
08 October 2018 7,497 3 View
Sandalwood oil is extracted from the heartwood. Is there a reason for or benefit from producing the oil in the heartwood?
14 February 2018 9,254 1 View
I'm trying to grow some E. coli strains in minimal medium containing free fatty acids. I've used Triton x-100 (1.5%) to disperse the fatty acids but bacteria didn't grow. I also tried adding the...
03 March 2017 3,767 9 View
I transformed BL21 and DH5a with a NAPE-AT plasmid. NAPE expression was similar in both strains. Now, I'm planning an animal study and need to use the strain that has better colonization or the...
01 February 2017 662 2 View
I'm growing 5637 cells for the first time. During splitting, I noticed that after 2 min in 1x trypsin-EDTA, the cells are still adhering to the bottom of the flask. Do I need to use a higher...
22 April 2015 235 7 View
I extracted a plant sample by soxhlet with chloroform for 8 h then methanol for another 8h. which way is better to fractionate the methanol extract, using sephadex or silica gel column? and what...
22 April 2015 3,018 11 View