How long can we consider that the data obtained with qualitative social research methods are "current"?

I cannot find any reference that deals with the temporal validity of qualitative data. I think there is a gap in the methodological literature on the temporal validity of the research results and,...

05 February 2021 9,788 20 View

Can I use the images of Collection 1 Level 2 Landsat 5 TM / Landsat 8 OLI to perform vegetation indices right away?

I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...

21 December 2020 4,088 3 View

BSEM/SEM approach?

Hi all! I'm getting into BSEM approach and I am specially concerned about when it is recommended or not to use this model. I have tested a BSEM with a small sample obtaining excellent results,...

07 September 2020 3,919 3 View

Unable to perfiorm raster to-table join in ArcGIS?

Dear all, I am trying to replicate some results from a publication. There, one of the datasets they use is WISE30sec map (available for download here:...

18 February 2020 6,906 3 View

Low 260/230 ratio using Qiagen DNeasy kit?

Recently I performed a DNA extraction using Qiagen DNeasy kit and the results show a 260/230 ratio of 1.05 likely due the presence of phenol or other organic contaminant... I have read that you...

16 February 2020 787 3 View

Why is the TMB substrate turning blue without being in contact with the sample and in the dark?

Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...

03 January 2020 1,294 7 View

How data is normalized in Transcriptome Analysis Console (TAC) Software?

I'm using TAC software 4.0.2 (Affimetrix) for the first time to analize differential gene expression in two groups of samples (control and treatment). I have problems with the normalization step...

18 November 2019 7,146 3 View

Importance of drying-time after using gelatin as coating?

I am culturing stem cells on plates coated with gelatin. After finding so many variations of drying time in different protocols, I am wondering what is the reason? In other words, why is it...

09 September 2019 9,415 3 View

How to interpret ordinal logistic regression in Stata with ordinal dependant and independent variables?

My question is very straightforward. I have different ordinal independent variables (4 categories), and I am trying to regress it on different ordinal and nominal variables. I want help on how I...

09 July 2019 5,769 1 View

Could you suggest any scale to assess parents' satisfaction with their children's schools?

Hi all! I'a looking for a scale to assess parents' satisfaction with their children's schools. I have found some different papers about parents satisfaction but no one seems to offer just what I...

10 December 2018 8,430 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View