I have a situation which quite do not understand. We are trying to get a qPCR to work. We extracted RNA and synthersized cDNA from have 5 Wts samples, 3 iRNA samples, and 1 genomic WT control. We are using primers that recognize the enter-exonicafusion of exons which means that any band must come from the cDNA and not from genomic DNA. We get the same band size using cDNA and genomic DNA but the mastermix control is clean. What can be going on? I appreciate any comment or suggestion