Patrick Gleason

2 Questions 10 Answers 0 Followers

Questions related from Patrick Gleason

Any advice on 3xFLAG elution from M2 Agarose Beads?

I have a FLAG-tagged fusion protein that I can successfully IP using Sigma's Anti-FLAG M2 Agarose Beads. This has been confirmed using S35-Methionine labeled fusion protein on numerous occasions....

10 October 2015 4,203 9 View

Why is my PCR product smaller than expected?

I am trying to clone eGFP + a linker into pCite4a. To do this, PCR was performed on a plasmid containing the eGFP with the linker built into the the reverse primer as such: Forward Primer: 5'...

07 July 2015 5,333 6 View