How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to smooth the energy production peaks of PV and wind energy?

Is power-to-gas a possible solution to this issue? Storing renewable energy in the form of H2 and CH4 and using them as fuels can increase the penetration of renewable energy in the transportation...

28 October 2020 3,490 3 View

Which are the most promising kinds of feedstock for renewable fuels?

Which kinds of biomass, byproducts and waste are the most interesting for new research development in anaerobic digestion and gasification?

03 May 2020 1,434 25 View

Power-to-gas from renewable energy sources: pros and cons?

Based on your expertise, what are advantages and disadvantages of producing H2 or CH4 from renewable sources (wind, PV etc)? I am mainly interested in collecting different points of view, e.g....

28 April 2020 1,926 8 View

Where do I find the Wilcox H test?

Hi all, I need to compare some population means, but my data are non-normally distributed and with unequal variance (even after transformation) so I can use neither parametric t-tests nor...

10 February 2020 6,485 7 View

Biohydrogen and dark fermentation: what is their potential?

Is the conversion rate a limiting factor of this technology, compared to gasification and hydrogenotrophic/acetoclastic methanogenesis? And does hydrogen have the chance to become a successful...

06 January 2020 3,349 5 View

Hoe can you cut hislogical section cuntaining calcium fragments??

Hello everybody, I have to cut histological sections cuntaining calcium fragments. Obviously the section are perforated at the bone fragments and I can't recover row sections. Do you have any...

30 July 2019 4,829 1 View

Is there a standard procedure to pre-process continuous blood pressure data?

Is there any standard Matlab pipeline/toolbox to preprocess continuous blood pressure (BP) data? I have collected BP and ECG data simultaneously and would like to know which filters I should use,...

14 March 2019 4,888 3 View

Could someone help me in the identification of this barnacle (Crustacea, Cirripedia)?

02 November 2016 3,927 4 View

Has anyone done a genetic transformation of tomato incorporating the gene for capsaicin from hot pepper (Capsicum annuum or C. chinense)?

14 May 2015 5,991 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View