I plan to treat C57BL/6J mice with a Western-type high-fat, high sugar diet for 16 weeks to induce obesity and NAFLD. I also plan to study ex-vivo central insulin resistance (Western blot in the hypothalamus). Doses of insulin range from 0.5 - 1 U/Kg BW to 5 U/mice in the published papers. I would appreciate receiving an expert opinion about the recommended insulin dose to be injected before sacrifice.