Cell membranes staining in whole-IHC in zebrafish larvae?

More Valerio Laghi's questions See All

How do results of Bland-Altman plots and regression analysis contribute to our understanding of the comparability between serum tubes and LIH tubes?

In the journal, "THE TRANSITION FROM GEL SEPARATORY SERUM TUBES TO LITHIUM HEPARIN GEL TUBES IN THE CLINICAL LABORATORY" by Oğuzhan Zengi, how do the results of Bland-Altman plots and regression...

09 April 2024 7,249 1 View

Which are the heat Interfaces between EPS and/or Batteries with Space Launcher vehicles?

For design a Modular Battery for Launchers I need to know the following information: 1. Which is the typical thermal environment of the launcher for the battery? 2. If it is foreseen a heat...

26 February 2024 644 0 View

260/230 ratio improvement on DNA samples?

Hello everyone, I'm currently having issues in trying to obtain an extracted DNA sample with 260/230 ratio values ranging from 2.0 to 2.2 for Nanopore downstream analysis. Right now after...

01 November 2023 5,609 0 View

NASA EIS Lithium Ion dataset: are phase measurments consistent?

We are analysing such dataset and it seems that such EIS measurements are not fully consistent. The phases are scattered and very limited in absolute value. The Magnitude values look a little...

02 March 2023 1,901 1 View

Is there a research about teaching reading?

Teaching reading to primary learners who doesn't know how to read

09 February 2023 3,862 3 View

How to measure Cohen's d (effect size) of ANCOVA, from either partial eta square or F-value?

I have a pre-post study, three groups, and one covariate. I have conducted two contrasts (1st group vs. 2nd+3rd; 2nd vs 3rd). The independent group is multicategorical, the dependent variable...

02 September 2022 5,789 5 View

Composition of the Binding Buffer from Dead Cell Removal Kit?

Hi, I would like to know if someone knows the composition of the Binding Buffer from the Dead Cell Removal Kit from Miltenyi Biotec. Have you tried a home-made alternative?

15 November 2021 4,803 1 View

What is the recommended insulin dose to be injected before sacrifice to assess a state of central insulin resistance in mice?

I plan to treat C57BL/6J mice with a Western-type high-fat, high sugar diet for 16 weeks to induce obesity and NAFLD. I also plan to study ex-vivo central insulin resistance (Western blot in the...

25 July 2021 1,432 1 View

Scale to measure science and spirituality beliefs?

Hi, I am looking for a scale to determine if participants are more prone to accept scientific or 'transcendental explanations to alternative therapies. My hypothesis is that if...

02 May 2021 8,471 5 View

Qiagen RNeasy 96 well too thick for centrifughe... alternatives?

Hello, I need to extract a big amount (hundred) of RNA from tissues and using single columns is no more feasible. We tried to use the Qiagen 96 well kit, because our samples are in RLT buffer...

31 March 2021 9,127 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View