Which is the best software to design nested PCR primers?

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Can 5' RLM RACE be used for quantification of miRNA cleavage products across samples?

I am using 5' RLM RACE to validate the targets of certain miRNAs in plants. I need to access the cleavage of particular targets among salt-resistant and salt-sensitive samples. Is it possible to...

11 December 2016 9,571 2 View

How to detect pre-miRNA levels in plants?

I want to detect the the levels of precursor miRNAs. Although there are many studies that explain detection of pre-miRNAs in animals, I have not been able to find many examples of the same in plants.

03 April 2016 4,359 2 View

Is it advisable to embed liver sections in paraffin for lipid staining ?

I need to determine lipid accumulation in liver. I plan to fix the tissue in 10% formalin. Should I embed the fixed tissue in paraffin, or cryopreserve the fixed tissue to prepare sections ?

10 November 2014 7,185 10 View

Has anyone used Fermentas RT enzyme ep0441?

We use fermentas RT enzyme (catalog number - ep0441) for reverse transcription. I was wondering if the cDNA formed is single-stranded or double stranded, as the enzyme has RNA-dependent and...

06 July 2014 1,616 1 View

How to obtain the entire sequence of pTZ57R/T for TA cloning?

I need to determine if there are any sites for the enzyme AscI in this vector for which I need the entire sequence. However, it does not seem to be available anywhere.

05 June 2014 9,783 5 View

What are the various possible ways of overexpressing microRNAs in vivo ?

I need to overexpress a microRNA in the livers of normal mice. Can mature miRNA mimics be used for this purpose? If yes, then what additional reagents are required?

05 June 2014 8,536 4 View

What is the mechanism of action of 4-PBA as a chemical chaperone ?

4-PBA is a commonly used to alleviate ER stress. I wanted to know how does it help in protein folding.

01 February 2014 8,933 18 View

Is there any non-radioactive method to check whether beta-oxidation pathway is inhibited ?

In my experiments, there is an accumulation of malonyl-CoA in HepG2 cells. I need to demonstrate whether this accumulation is inhibiting beta-oxidation pathway or not.

10 November 2013 8,594 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View