How to obtain the entire sequence of pTZ57R/T for TA cloning?

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J.A. Curiel

Hi Himanshi! You can try looking this... Notice that is talking about pTZ57R, but the sequence is the same than your plasmid.

http://www.thermoscientificbio.com/uploadedFiles/Resources/pTZ57R-map.pdf

Himanshi Bhatia

hey thanx guys, but i do have these files. However, this sheet does not mention AscI enzyme, neither in the cutter list nor in the non-cutter list.

Sarju Patel

its available at

http://www.thermoscientificbio.com/molecular-cloning/instaclone-pcr-cloning-kit/

Ok Himanshi! You have to go at this website:

Then search the "includes" part, there you can found (pTZ57R Sequence in FASTA format). In this way you can get the completed sequence of your plasmid!!

Good luck!

PD: I saw in gene runner that Asc I doesn`t cut...

thanks for the help !!!