Himanshi Bhatia

9 Questions 16 Answers 0 Followers

Questions related from Himanshi Bhatia

Can 5' RLM RACE be used for quantification of miRNA cleavage products across samples?

I am using 5' RLM RACE to validate the targets of certain miRNAs in plants. I need to access the cleavage of particular targets among salt-resistant and salt-sensitive samples. Is it possible to...

12 December 2016 9,556 2 View

Which is the best software to design nested PCR primers?

I have to perform 5' RLM-RACE for target validation of chickpea miRNAs. Which primer design softwares have the option for designing primers for nested PCR?

07 July 2016 2,914 3 View

How to detect pre-miRNA levels in plants?

I want to detect the the levels of precursor miRNAs. Although there are many studies that explain detection of pre-miRNAs in animals, I have not been able to find many examples of the same in plants.

04 April 2016 4,343 2 View

Is it advisable to embed liver sections in paraffin for lipid staining ?

I need to determine lipid accumulation in liver. I plan to fix the tissue in 10% formalin. Should I embed the fixed tissue in paraffin, or cryopreserve the fixed tissue to prepare sections ?

11 November 2014 7,168 10 View

Has anyone used Fermentas RT enzyme ep0441?

We use fermentas RT enzyme (catalog number - ep0441) for reverse transcription. I was wondering if the cDNA formed is single-stranded or double stranded, as the enzyme has RNA-dependent and...

07 July 2014 1,599 1 View

What are the various possible ways of overexpressing microRNAs in vivo ?

I need to overexpress a microRNA in the livers of normal mice. Can mature miRNA mimics be used for this purpose? If yes, then what additional reagents are required?

06 June 2014 8,520 4 View

How to obtain the entire sequence of pTZ57R/T for TA cloning?

I need to determine if there are any sites for the enzyme AscI in this vector for which I need the entire sequence. However, it does not seem to be available anywhere.

06 June 2014 9,761 5 View

What is the mechanism of action of 4-PBA as a chemical chaperone ?

4-PBA is a commonly used to alleviate ER stress. I wanted to know how does it help in protein folding.

02 February 2014 8,913 18 View

Is there any non-radioactive method to check whether beta-oxidation pathway is inhibited ?

In my experiments, there is an accumulation of malonyl-CoA in HepG2 cells. I need to demonstrate whether this accumulation is inhibiting beta-oxidation pathway or not.

11 November 2013 8,577 1 View