Hello Himanshi,

4-PBA is supposed to interact with hydrophobic domains of mis-folded proteins and thus prevents their aggregation. This way the chances for correct folding of the proteins to get their right conformation is increased.

Best regards

Hello Himanshi,

4-PBA is supposed to interact with hydrophobic domains of mis-folded proteins and thus prevents their aggregation. This way the chances for correct folding of the proteins to get their right conformation is increased.

Best regards

Thanks !

Hello what is the stock concentration of your PBA ? I would like to prepare the stock and treat cells with it to alleviate ER stress.

I prepare 1M stock solution of 4-PBA (using NaOH to dissolve 4-PBA in water) and the final working conc. of 4-PBA is 1mM or 5mM. I have used these concentrations to revert ER stress in HepG2 cells.

Thank you very much Himanshi. Does the pH of NaoH matter? should I use 7.54 or 8?

Just a concentrated solution of NaOH will do fine.

Thank you once again Himanshi. Now I have question on pre-treatment. what does pre-trearment mean?  Do I treat the cells with PBA for, lets say 4 hours, then add ER stress inducers in addation to already added PBA or after 4 hours I replace the 4PBA+media with media +ER stress inducers?

Hi, apologies for the late reply...

I first treat my cells with the ER -stress inducing factor, then add 4-PBA.

Himanshi, did we have to use NAOH to dissolve it? Thanks

Hi everybody.

I would like to use 4-PBA soon. How do you store the 1M solution (dissolved in water?). Thanks!!

Yes, I used NaOH to dissolve it. The solution was stored at 4 deg Celsius for short-term storage (max. one month); I prepared fresh solution after that.

Hello everybody,

we are having some problems trying to make a stock solution of 4-PBA. We can dissolve it in water when we add the NaOH, but it precipitates again when we adjust the pH adding HCl. Can you please tell us how you managed to make your 1M stock solution?  Thanks!

Dear All,

I realize this is quite late, but I have been having trouble preparing a stock solution of 4PBA. Despite my best efforts, and repeated heating etc. the crystals never seem to fully dissolve. Could I maybe ask if somebody could offer some insight into the exact protocol they used to prepare the stock solution? e.g. titration with NaOH, the solvent used, and the final pH it was used at?

I would appreciate this very much

Thank You

Buy the sodium salt. Dissolved in MEM, like a charm...

Hey Mark, thank you for your response. I also purchased the solid form from Sigma, but despite this I am having trouble. The data sheets lists only water and DMSO as compatible solvents. Do you mean MEM as in the media? If so could you tell me what concetration of stock solution you prepared and any additional steps you took?

Hello Nayana

Were you able to figure out how to dissolve the solid PBA from sigma? Our lab is runnign into similar problems- Water and NaOH work to dissolve the solvent, but the amount of NaOH we use makes the solution extremely basic- what pH did your solution come out to?

Also, does anyone have any insight into how heat-tolerant the PBA is? We will be adding the solution to drosophila food at 70 C, and arent sure if this will degrade the PBA

thank you!

Hi all, in continuation of this thread I would like to have to know how 4PBA enters into the cells? Is it through caveolin-mediated endocytosis or through micropinocytosis or through clathrin-mediated endocytosis?