Cardiovascular research and its shortcomings?

What are the important cardiovascular research areas? and why is it that after so many decades of prevalent research in this area, it is still the number one cause of death? What are the...

02 March 2016 372 0 View

Why does my protein not bind to the DEAE column at pH 7 but it does at pH 8?

the pI of my protein is 5.28. while purifying it when I use buffer of pH 7.2 the protein does not bind to DEAE column but when I use buffer of pH 8 it does. What could be the possible reasons for...

03 April 2015 1,436 15 View

While applying for Ph.D, what all things should we look for and choose the most appropriate lab?

India as well as abroad

11 December 2014 9,218 0 View

Which is the best company for plasmid DNA sequencing?

I have tried sending my samples to one and it has only been a waste of time. Any suggestions?

11 December 2014 5,059 3 View

How do we go about sequencing data analysis after SDM?

11 December 2014 5,502 1 View

Why do I get no colonies after transformation after SDM?

What could be the reasons for not getting colonies on transformation into E.coli competent after Side directed mutagenesis? There is no problem with DpnI digestion, competent cells, antibiotic...

11 December 2014 6,241 6 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View