How do we go about sequencing data analysis after SDM?

12 December 2014 1 6K Report

I have done one amino acid mutation using SDM, and had sent the transformants for sequencing using self designed primer. Now I am a little confused as to how to analyze it.

Breanna Symmes

If you have a sequencing program like MacVector, you can generate a sequence file for what the sequence should (ideally) look like after SGM, then input the sequencing results directly into the program and compare them. If you do not have such a program, you could also do a BLAST comparison.

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