Adi Jindal

7 Questions 11 Answers 0 Followers

Questions related from Adi Jindal

Cardiovascular research and its shortcomings?

What are the important cardiovascular research areas? and why is it that after so many decades of prevalent research in this area, it is still the number one cause of death? What are the...

03 March 2016 408 0 View

Cardiac fibroblast specific markers?

I need to check for fibroblasts isolated from rat heart, what all markers are exclusively specific to cardiac fibroblast? I need to do western blot and immunostaining using confocal...

02 February 2016 8,021 2 View

Why does my protein not bind to the DEAE column at pH 7 but it does at pH 8?

the pI of my protein is 5.28. while purifying it when I use buffer of pH 7.2 the protein does not bind to DEAE column but when I use buffer of pH 8 it does. What could be the possible reasons for...

04 April 2015 1,553 15 View

While applying for Ph.D, what all things should we look for and choose the most appropriate lab?

India as well as abroad

12 December 2014 9,257 0 View

How do we go about sequencing data analysis after SDM?

I have done one amino acid mutation using SDM, and had sent the transformants for sequencing using self designed primer. Now I am a little confused as to how to analyze it.

12 December 2014 5,547 1 View

Why do I get no colonies after transformation after SDM?

What could be the reasons for not getting colonies on transformation into E.coli competent after Side directed mutagenesis? There is no problem with DpnI digestion, competent cells, antibiotic...

12 December 2014 6,337 6 View

Which is the best company for plasmid DNA sequencing?

I have tried sending my samples to one and it has only been a waste of time. Any suggestions?

12 December 2014 5,135 3 View