Cardiac fibroblast specific markers?

I need to check for fibroblasts isolated from rat heart, what all markers are exclusively specific to cardiac fibroblast? I need to do western blot and immunostaining using confocal...

01 February 2016 7,980 2 View

Why does my protein not bind to the DEAE column at pH 7 but it does at pH 8?

the pI of my protein is 5.28. while purifying it when I use buffer of pH 7.2 the protein does not bind to DEAE column but when I use buffer of pH 8 it does. What could be the possible reasons for...

03 April 2015 1,436 15 View

While applying for Ph.D, what all things should we look for and choose the most appropriate lab?

India as well as abroad

11 December 2014 9,218 0 View

Which is the best company for plasmid DNA sequencing?

I have tried sending my samples to one and it has only been a waste of time. Any suggestions?

11 December 2014 5,059 3 View

How do we go about sequencing data analysis after SDM?

11 December 2014 5,502 1 View

Why do I get no colonies after transformation after SDM?

What could be the reasons for not getting colonies on transformation into E.coli competent after Side directed mutagenesis? There is no problem with DpnI digestion, competent cells, antibiotic...

11 December 2014 6,241 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View