I am planning to use CRISPR/Cas9 to endogenously label my protein of interest with a tag, I then want to use these cells (U2OS cell line) for imaging.
Would it be better to use a fluorescent tag (like Halo/SNAP), which can then be useful to FACS-sort successfully edited cells displaying fluorescence. Disadvantage being that the these tags are quite large.
Or would it be better to use a smaller protein-tags like V-5 and then use antibiotic resistance and PCR-analysis to identify successfully edited cells.
I haven’t used CRISPR knockin before and wanted inputs from the community.
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