I have 50 ug of Human Her2 / ErbB2 Protein, Tag Free antigen in white powder form and need to make various concentrations of that antigen, such as 50 unit/ml, 10 unit/ml, 0.01 unit/ml, and 0.1 unit/ml. How can I do that? How can I make different antigen concentrations using a 50 ug stock size? What is the calculation for that?
Hi Shruti,
You may use the web tool to calculate
https://www.alfa-chemistry.com/solution-dilution-calculator.htm
https://www.alfa-chemistry.com/mass-molarity-calculator.htm
Hi Shruti,
You need to find its specific activity else you can't convert. The specific activity may be written on the label or in the product information sheet or certificate analysis for the particular lot of the product that you received.
All the best!
Vipul Batra Hello sir, But They didn't mention a specific activity on the label, how can I find that? is specific activity given in Unit/ml or Unit/ul?
Hi Shruti Deshpande It can be either. Would you mind sharing the catalogue and details of the product? Most often it is mentioned in the Certificate of Analysis.
Vipul Batra Hello sir, I have share with you COA of the mentioned product
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