Which is more efficient for CRISPR knockin - using a smaller tag like V5 vs using self-labeling tags like Halo?

I am planning to use CRISPR/Cas9 to endogenously label my protein of interest with a tag, I then want to use these cells (U2OS cell line) for imaging. Would it be better to use a fluorescent tag...

08 March 2022 1,211 0 View