Is there any role of phenolic elicitors like phloroglucinol in inducing or reducing clonal variability in micropropagated plants?

I have used phloroglucinol in inducing higher root induction in various species of medicinal orchids primarily dendrobes. In a recent study, I have found using different classes of cytokinins...

05 June 2016 9,777 2 View

What is the standard DNA-barcode gene for medicinal plants, especially orchids?

I have come across certain papers where ITS-2 is claimed as the standard DNA barcode gene. Apart from ITS-2 is there any other gene which can be used? If yes, why so? I am enclosing one such...

07 August 2014 5,677 4 View

What is the latest molecular marker technique for genetic diversity analysis?

I want to use some new molecular marker techniques apart from these i.e. RAPD, ISSR, DAMD, SCoT, SSR, ITAP and ISJ. Excluding these 7 markers, can anybody name any other molecular marker which can...

04 May 2014 9,797 5 View

Does the ploidy level of a species govern the patterns of gene expression in a plant species?

How is ploidy linked with gene expression of various significant genes such as PAL, troponine reductase, etc?

03 April 2014 8,813 1 View

How to extract DNA from silica gel preserved leaf samples especially orchids?

I want to extract DNA from silica gel preserved leaf samples of Orchids (dendrobiums). As orchids are having mucilaginous leaf tissue its difficult to extract DNA (my experience). If I dehydrate...

31 December 2013 7,106 15 View

What is the HPLC standard for various compounds in Dendrobium nobile orchid?

Dendrobium nobile houses a variety of chemical compounds in it. Some of them are dendrobine, moscatilin, gigantol etc. While I am searching for HPLC standards for dendrobine, I am getting these...

31 December 2013 9,708 6 View

I want the promoter sequence of Acetyl CoA carboxylase gene of Oryza sativa. Any suggestions how to get Acetyl CoA carboxylase gene of Oryza sativa?

I have searched NCBI for this particular sequence. But I am not getting anything. I think I am making some mistakes in the search. Can anybody please help me?

31 December 2013 312 6 View

What is IMPACT INDEX? is it a valid parameter?

I have come across a journal which is showing a ISJN number and an impact index of 4.63. Now is this a valid score? The journal url is...

31 December 2013 8,412 1 View

Can GENE SCAN analysis using automated DNA sequencer be applied for analysis of SRAP molecular marker data?

I have came across papers where people have used GENE SCAN analysis to analyse AFLP marker datas. This feature is present in all ABI automated DNA sequencers. Can this approach be applied in case...

31 December 2013 5,275 2 View

Could the explants derived from Protocorm-like bodies or Protocorms in orchids be called "Cotyledonary explants"?

Orchids are monocots. After asymbiotic germination protocorms are produced. If we use these protocorms as explant or protocorm, derived plantlets as explant source, can we designate them as...

09 October 2013 781 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View