01 January 2014 2 5K Report

I have came across papers where people have used GENE SCAN analysis to analyse AFLP marker datas. This feature is present in all ABI automated DNA sequencers. Can this approach be applied in case of other molecular markers?

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Is there any role of phenolic elicitors like phloroglucinol in inducing or reducing clonal variability in micropropagated plants?

I have used phloroglucinol in inducing higher root induction in various species of medicinal orchids primarily dendrobes. In a recent study, I have found using different classes of cytokinins...

05 June 2016 9,777 2 View

What is the standard DNA-barcode gene for medicinal plants, especially orchids?

I have come across certain papers where ITS-2 is claimed as the standard DNA barcode gene. Apart from ITS-2 is there any other gene which can be used? If yes, why so? I am enclosing one such...

07 August 2014 5,677 4 View

What is the latest molecular marker technique for genetic diversity analysis?

I want to use some new molecular marker techniques apart from these i.e. RAPD, ISSR, DAMD, SCoT, SSR, ITAP and ISJ. Excluding these 7 markers, can anybody name any other molecular marker which can...

04 May 2014 9,797 5 View

Does the ploidy level of a species govern the patterns of gene expression in a plant species?

How is ploidy linked with gene expression of various significant genes such as PAL, troponine reductase, etc?

03 April 2014 8,813 1 View

How to extract DNA from silica gel preserved leaf samples especially orchids?

I want to extract DNA from silica gel preserved leaf samples of Orchids (dendrobiums). As orchids are having mucilaginous leaf tissue its difficult to extract DNA (my experience). If I dehydrate...

31 December 2013 7,106 15 View

What is the HPLC standard for various compounds in Dendrobium nobile orchid?

Dendrobium nobile houses a variety of chemical compounds in it. Some of them are dendrobine, moscatilin, gigantol etc. While I am searching for HPLC standards for dendrobine, I am getting these...

31 December 2013 9,708 6 View

I want the promoter sequence of Acetyl CoA carboxylase gene of Oryza sativa. Any suggestions how to get Acetyl CoA carboxylase gene of Oryza sativa?

I have searched NCBI for this particular sequence. But I am not getting anything. I think I am making some mistakes in the search. Can anybody please help me?

31 December 2013 312 6 View

What is IMPACT INDEX? is it a valid parameter?

I have come across a journal which is showing a ISJN number and an impact index of 4.63. Now is this a valid score? The journal url is...

31 December 2013 8,412 1 View

Could the explants derived from Protocorm-like bodies or Protocorms in orchids be called "Cotyledonary explants"?

Orchids are monocots. After asymbiotic germination protocorms are produced. If we use these protocorms as explant or protocorm, derived plantlets as explant source, can we designate them as...

09 October 2013 781 2 View

Which growth regulator combination or individual is best to raise/initiate cultures from ex vivo explant source in orchids?

I want to establish cultures of certain dendrobes from ex vivo source.Can you please suggest the PGRs whose combinations/individuals are ideal?

08 September 2013 3,274 7 View

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