Can GENE SCAN analysis using automated DNA sequencer be applied for analysis of SRAP molecular marker data?

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Is there any role of phenolic elicitors like phloroglucinol in inducing or reducing clonal variability in micropropagated plants?

I have used phloroglucinol in inducing higher root induction in various species of medicinal orchids primarily dendrobes. In a recent study, I have found using different classes of cytokinins...

05 June 2016 9,847 2 View

What is the standard DNA-barcode gene for medicinal plants, especially orchids?

I have come across certain papers where ITS-2 is claimed as the standard DNA barcode gene. Apart from ITS-2 is there any other gene which can be used? If yes, why so? I am enclosing one such...

07 August 2014 5,786 4 View

What is the latest molecular marker technique for genetic diversity analysis?

I want to use some new molecular marker techniques apart from these i.e. RAPD, ISSR, DAMD, SCoT, SSR, ITAP and ISJ. Excluding these 7 markers, can anybody name any other molecular marker which can...

04 May 2014 9,881 5 View

Does the ploidy level of a species govern the patterns of gene expression in a plant species?

How is ploidy linked with gene expression of various significant genes such as PAL, troponine reductase, etc?

03 April 2014 8,898 1 View

How to extract DNA from silica gel preserved leaf samples especially orchids?

I want to extract DNA from silica gel preserved leaf samples of Orchids (dendrobiums). As orchids are having mucilaginous leaf tissue its difficult to extract DNA (my experience). If I dehydrate...

31 December 2013 7,202 15 View

What is the HPLC standard for various compounds in Dendrobium nobile orchid?

Dendrobium nobile houses a variety of chemical compounds in it. Some of them are dendrobine, moscatilin, gigantol etc. While I am searching for HPLC standards for dendrobine, I am getting these...

31 December 2013 9,795 6 View

What is IMPACT INDEX? is it a valid parameter?

I have come across a journal which is showing a ISJN number and an impact index of 4.63. Now is this a valid score? The journal url is...

31 December 2013 8,498 1 View

I want the promoter sequence of Acetyl CoA carboxylase gene of Oryza sativa. Any suggestions how to get Acetyl CoA carboxylase gene of Oryza sativa?

I have searched NCBI for this particular sequence. But I am not getting anything. I think I am making some mistakes in the search. Can anybody please help me?

31 December 2013 391 6 View

Could the explants derived from Protocorm-like bodies or Protocorms in orchids be called "Cotyledonary explants"?

Orchids are monocots. After asymbiotic germination protocorms are produced. If we use these protocorms as explant or protocorm, derived plantlets as explant source, can we designate them as...

09 October 2013 873 2 View

Which growth regulator combination or individual is best to raise/initiate cultures from ex vivo explant source in orchids?

I want to establish cultures of certain dendrobes from ex vivo source.Can you please suggest the PGRs whose combinations/individuals are ideal?

08 September 2013 3,352 7 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View