Paromik Bhattacharyya

19 Questions 17 Answers 0 Followers

Questions related from Paromik Bhattacharyya

Is there any role of phenolic elicitors like phloroglucinol in inducing or reducing clonal variability in micropropagated plants?

I have used phloroglucinol in inducing higher root induction in various species of medicinal orchids primarily dendrobes. In a recent study, I have found using different classes of cytokinins...

06 June 2016 9,819 2 View

What is the standard DNA-barcode gene for medicinal plants, especially orchids?

I have come across certain papers where ITS-2 is claimed as the standard DNA barcode gene. Apart from ITS-2 is there any other gene which can be used? If yes, why so? I am enclosing one such...

08 August 2014 5,740 4 View

What is the latest molecular marker technique for genetic diversity analysis?

I want to use some new molecular marker techniques apart from these i.e. RAPD, ISSR, DAMD, SCoT, SSR, ITAP and ISJ. Excluding these 7 markers, can anybody name any other molecular marker which can...

05 May 2014 9,845 5 View

Does the ploidy level of a species govern the patterns of gene expression in a plant species?

How is ploidy linked with gene expression of various significant genes such as PAL, troponine reductase, etc?

04 April 2014 8,861 1 View

How to extract DNA from silica gel preserved leaf samples especially orchids?

I want to extract DNA from silica gel preserved leaf samples of Orchids (dendrobiums). As orchids are having mucilaginous leaf tissue its difficult to extract DNA (my experience). If I dehydrate...

01 January 2014 7,163 15 View

What is IMPACT INDEX? is it a valid parameter?

I have come across a journal which is showing a ISJN number and an impact index of 4.63. Now is this a valid score? The journal url is...

01 January 2014 8,459 1 View

Can GENE SCAN analysis using automated DNA sequencer be applied for analysis of SRAP molecular marker data?

I have came across papers where people have used GENE SCAN analysis to analyse AFLP marker datas. This feature is present in all ABI automated DNA sequencers. Can this approach be applied in case...

01 January 2014 5,339 2 View

I want the promoter sequence of Acetyl CoA carboxylase gene of Oryza sativa. Any suggestions how to get Acetyl CoA carboxylase gene of Oryza sativa?

I have searched NCBI for this particular sequence. But I am not getting anything. I think I am making some mistakes in the search. Can anybody please help me?

01 January 2014 354 6 View

What is the HPLC standard for various compounds in Dendrobium nobile orchid?

Dendrobium nobile houses a variety of chemical compounds in it. Some of them are dendrobine, moscatilin, gigantol etc. While I am searching for HPLC standards for dendrobine, I am getting these...

01 January 2014 9,758 6 View

Could the explants derived from Protocorm-like bodies or Protocorms in orchids be called "Cotyledonary explants"?

Orchids are monocots. After asymbiotic germination protocorms are produced. If we use these protocorms as explant or protocorm, derived plantlets as explant source, can we designate them as...

10 October 2013 839 2 View

How to detect/check plagiarism in a research manuscript?

Suppose I want to check an MS for plagiarism which has come for reviewing to me. What is the standard procedure?

09 September 2013 251 25 View

Which growth regulator combination or individual is best to raise/initiate cultures from ex vivo explant source in orchids?

I want to establish cultures of certain dendrobes from ex vivo source.Can you please suggest the PGRs whose combinations/individuals are ideal?

09 September 2013 3,318 7 View

How genetic diversity in a plant species can be corelated with molecular marker datas using in silico approach?

We have performed molecular analysis of certain endangered plant species using molecular marker datas. My question is there any methodology/protocol available by which this datas can be corelated...

08 August 2013 9,269 3 View

Why in crosspolinated plants genetic variability is more in seed raised plants than in invivo explant raised plantlets?

I have seen in my experiments (orchids) that the rate of variability is much higher when i am germinating a orchid pod and then geeting the plantlets and afterwards extracting DNA from the raised...

08 August 2013 8,759 1 View

After transformation using agarobacterium in legumes I am having problems in rooting. What may be the reason?

I am facing problems with transformation after successful transformation of the plant. But when I am introducing the plants for climatic adaptation the roots are dying. What may be the solution?

07 July 2013 4,402 3 View

Is it possible to link up genetic diversity with expressibility and production of a particular secondary metabolite in a plant species?

Suppose we are studying the diversity of a plant population group. It has distributions in various terrains. But after analysis of both diversity data and biochemical data of a particular...

07 July 2013 1,265 9 View

Do natural extracts like coconut water have lesser possibility to give rise to somoclonal variations than synthetic PGRs?

I have compared clones raised from both coconut water and synthetic PGRs. When I checked the fidelity of the hardened plants where I used coconut water as the additive it gave a lesser degree of...

07 July 2013 4,957 3 View

Is there any software package available which we can use for scoring DNA bands?

04 April 2013 8,638 8 View

Can anybody suggest what is the best and comprehensive process for analysing/detecting genetic stability/fidelity within micropropagated plants?

Genetic Stability analysis

04 April 2013 6,550 5 View