Is there any role of phenolic elicitors like phloroglucinol in inducing or reducing clonal variability in micropropagated plants?

I have used phloroglucinol in inducing higher root induction in various species of medicinal orchids primarily dendrobes. In a recent study, I have found using different classes of cytokinins...

05 June 2016 9,777 2 View

What is the standard DNA-barcode gene for medicinal plants, especially orchids?

I have come across certain papers where ITS-2 is claimed as the standard DNA barcode gene. Apart from ITS-2 is there any other gene which can be used? If yes, why so? I am enclosing one such...

07 August 2014 5,677 4 View

What is the latest molecular marker technique for genetic diversity analysis?

I want to use some new molecular marker techniques apart from these i.e. RAPD, ISSR, DAMD, SCoT, SSR, ITAP and ISJ. Excluding these 7 markers, can anybody name any other molecular marker which can...

04 May 2014 9,797 5 View

Does the ploidy level of a species govern the patterns of gene expression in a plant species?

How is ploidy linked with gene expression of various significant genes such as PAL, troponine reductase, etc?

03 April 2014 8,813 1 View

How to extract DNA from silica gel preserved leaf samples especially orchids?

I want to extract DNA from silica gel preserved leaf samples of Orchids (dendrobiums). As orchids are having mucilaginous leaf tissue its difficult to extract DNA (my experience). If I dehydrate...

31 December 2013 7,106 15 View

I want the promoter sequence of Acetyl CoA carboxylase gene of Oryza sativa. Any suggestions how to get Acetyl CoA carboxylase gene of Oryza sativa?

I have searched NCBI for this particular sequence. But I am not getting anything. I think I am making some mistakes in the search. Can anybody please help me?

31 December 2013 312 6 View

What is IMPACT INDEX? is it a valid parameter?

I have come across a journal which is showing a ISJN number and an impact index of 4.63. Now is this a valid score? The journal url is...

31 December 2013 8,412 1 View

Can GENE SCAN analysis using automated DNA sequencer be applied for analysis of SRAP molecular marker data?

I have came across papers where people have used GENE SCAN analysis to analyse AFLP marker datas. This feature is present in all ABI automated DNA sequencers. Can this approach be applied in case...

31 December 2013 5,275 2 View

Could the explants derived from Protocorm-like bodies or Protocorms in orchids be called "Cotyledonary explants"?

Orchids are monocots. After asymbiotic germination protocorms are produced. If we use these protocorms as explant or protocorm, derived plantlets as explant source, can we designate them as...

09 October 2013 781 2 View

Which growth regulator combination or individual is best to raise/initiate cultures from ex vivo explant source in orchids?

I want to establish cultures of certain dendrobes from ex vivo source.Can you please suggest the PGRs whose combinations/individuals are ideal?

08 September 2013 3,274 7 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View