I looking for a brief resource about comparison between ZFN, TALEN, CRISPR/Cas system of genome editing. Actually I'm interested in their merits and demerits.
It depends on the organism you are working with and what you want to do, just NHEJ or HR? There are several reviews out these days.
I would prefere CRISPR/Cas9 because it is cheap, easy and very efficient and constructs for various organims exist.
You need ~ 2 weeks for the creation of a TALEN pair and at least 10 different Vectors which you need to reassamble. When doing CRISPR/Cas9 you need 2 Vektors and an Oligo thats all!
If you need more information I would recommend some reviews,
recent ones in Current opinion on biotech about TALENs and one about CRISPR
http://www.sciencedirect.com/science/journal/09581669/32
Cheers
Thorben
Agreed with Thorben -- CRISPR is so much more convenient that it's pretty much the only game in town, unless you can draw on a specialized team with lots of preexisting resources and expertise.
It depends on your purpose, if you want to do knock out or silencing to validate candidate genes then I would suggest using CRISPPR because it is cheap and faster.
But if you want to do gene insertion ( a huge sequence) then I would suggest to use ZFNs or TALENs, even though they are a little complicated to build but they are much precise in delivering your gene of interest to the insertion site unlike with Crisprs that you would experience off targets of your GOI. Hope it helps!
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