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Questions related from Christian Cantos
I know that the cut or the DSB happened 5-6bp away from the PAM sequence, inside the guided RNA spacer sequence. But is it possible that the mutation occurred not in the guided RNA spacer...
23 April 2015 4,001 1 View
I am analyzing samples transfected by CRISPR using the T7 assay. And when I am trying to analyze it on the gel, I can found smears on my samples. I don't know if I added too much enzyme or very...
27 March 2015 4,062 3 View
What method can you suggest to determine if the mutation created by the CRISPR technology is mono or bi allelic?
26 March 2015 8,349 13 View
Initially, I did Agrobacterium transformation with my constructs and gave many colonies. Then did PCR and sequencing for several colonies and showed that my constructs were transformed. Then I re...
16 December 2014 4,832 8 View
We are doing this assay, where we want to detect certain activity of an enzyme to degrade starch. One of the parameters also is to check for total sugar content of the enzyme reaction. What can...
21 November 2014 2,340 6 View
I am trying to put together the promoter, gene and terminator in pUC19 backbone. I followed the protocol of NEB in the Gibson mix. But I am not getting any colonies in my plate ( even blue...
10 July 2014 6,831 4 View
I am designing a guide RNA for Crispr to target specific genes in rice that when silenced can have a good effect on the stability of rice, such as disease-resistance.
11 June 2014 7,761 2 View
