Can I perform FACS doublet discrimination in a heterogenous population of cells?

My samples are a mix of cancer cells and lymphocytes. I usually do doublets discrimination when performing data analysis but I would like to know if a heterogeneous doublet formed by a cancer cell...

03 November 2018 7,091 5 View

Which are the main research gaps in Water Harvesting?

Dear all, this is a major question and I hope to start a fruitful discussion. Which are, in your view, the main research gaps in the field of water harvesting? Would you mind to provide recent...

21 December 2017 8,178 11 View

Which MATLAB equation solver is more suitable?

I have a system of 16 variables of non linear and non-smooth equations (there are some min/max functions).Which solver is more suitable?

21 January 2017 3,859 4 View

What is the best way to interpolate raingauges data over mountain areas?

08 August 2016 8,844 8 View

Does anyone know a way to convert the World Reference Base for Soil Resources to the hydrological soil group?

Does anyone know a way to convert the World Reference Base for Soil Resources classification to the hydrological soil group? In particular I would need a conversion table to SWAT soil classification

21 July 2016 2,742 7 View

Hydrology and Water Management Research in Mozambique

Dear all, I am actually in Mozambique working on a rural development project. My department is actually involved as partner to do assessment and design activities, but we would love to insert some...

01 January 1970 6,700 3 View

How can I label an antibody to determine how far it is travelling from the microdialysis probe?

I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...

09 February 2021 3,981 1 View

Fluorophore panel selection?

Hello, I'm trying to select fluorophores for a large panel of flow cytometry, so I'm wondering what is the maximum degree of overlap between two fluorophores that is considered acceptable? Thanks.

19 January 2021 3,475 3 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Suitable secondary standard for Raman spectroscopic intensity profile correction at 785 nm excitation?

Once the intensity profile of a 785 nm excitation Raman spectrometer has been standardised, e.g. using either white light or NIST SRM-2241, these can be routinely monitored using the same primary...

06 December 2020 2,807 1 View

How to check spread of AAV2 virus that doesn't express fluorophores?

Hi, I am injecting AAV2-Retro virus (AAV2-retro-EF1a-DIO-FLPo-WPRE-hGHpA from Addgene) that carries cre-dependent flp recombinase, in the mouse dorsal hippocampus and I need to verify that the...

08 November 2020 9,559 3 View

How to increase whiteness index of PVC with Visible light photobleaching?

Dear All, As I know, PVC color is affected by conjugated double bond amount in the PVC structure and I have difficulties to find the optimum condition to rearrange or decrease the conjugated...

14 October 2020 662 1 View

What's the best fluorophore to use for KI67 analysis?

I would like to check proliferation of human CD8 cells (isolated from blood ) after few days of culture. I have a panel of antibodies and I want to add KI67 to analyse proliferation. Does anyone...

08 October 2020 4,763 3 View

Can a RFU plot have no Ct value in a qPCR reaction?

Hi, We have a small test run for a set of RNA samples with 3 fluorophores, FAM, HEX, and Cy5. FAM and HEX are for test samples while Cy5 is with internal control. Surprisingly, for some of our...

03 September 2020 7,713 3 View

Do you have any other suggestion to reduce autofluorescence in FFPE tissue?

I used Fluorophore Conjugated primary antibody (Alexa-488, 555 and 647). Tissue FFPE samples were imaged before I stainded antibody and I found background fluorescence. So, my question is "what...

18 August 2020 2,294 3 View

Is it possible to carryout Chlorophyllin (Mg-Chl, or Cu-Chl) photosensitized degradation of methylene blue dye in presence of visible light ?

As both Chlorophyll semi-synthetic derivative and methylene blue organic dye has been widely used as a potential photosensitizer in applications like PDT but I want to know that; a chlorophyll...

15 August 2020 6,325 3 View