I am starting out on some siRNA based knockdowns on primary cells. Would anyone recommend good services they have used in India and any advice considering the advantage of modified and fluorophore conjugated siRNAs from your experience..
I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...
17 February 2021 384 3 View
I'm familiar with Geochemical data analysis software such as GCDKit, PetroGraph, and Petroplot etc. which one is best and suitable for doing Geochemical Modelling of Igneous Processes? I need...
22 December 2020 9,300 2 View
I want to create a large scale land use land cover change map of a village of India in the 10-year interval. Which one is the appropriate satellite image for this. and anybody kindly explains the...
12 October 2020 3,996 6 View
I have performed In-vivo study in rate model for new formulation as well as marketed formulation. Based on results new formulation have significant improvement in all PK parameters. Oral dose...
20 August 2020 2,209 1 View
i want information on fluorescence based experiments
17 February 2020 1,805 1 View
I am doing a research in Cement Industry with emphasis on perceived employee performance management. I am looking for the measuring scale of the same.
09 January 2020 6,363 3 View
I am trying to separate lactic acid via distillation, from the bio-crude I got after performing hydro-thermal liquefaction of biomass. however, it seemed very challenging as always other organic...
07 January 2020 9,265 1 View
I was successful with the transformation( pPIC9K vector in Pichia pastoris GS115 strain )and got good colonies in RDB plates after 48 hours. later in MD and MM plates also. Can anyone please tell...
09 December 2019 6,346 8 View
i want to prepare some sulfa drugs ophthalmic formulation
05 December 2019 8,017 2 View
Hi, I'm working on sequence alignment algorithms. My background is Computer Science. Given two sequences, what could be the max length of a gap and how many insertions/deletion at one stretch I...
16 November 2019 1,767 10 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...
25 February 2021 9,659 2 View
How to conjugate two acids with amine linker?
18 February 2021 4,083 2 View
I am just so confused that some people tell me knockdown is easier than overexpression when generating a stable cell line. I think it depends on the gene. Cells won't be happy whether we try to...
15 February 2021 1,370 2 View
i am having a problem with the transfection of 2 siRNA , one for the longer isoform U and the other one of the sliced isoform S that is also the only transcribed. i am trasfection siRNA with a...
11 February 2021 8,145 3 View
I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....
11 February 2021 6,558 2 View
Hi everyone. I am working on metal oxides. I need to make some conjugates of drugs and NP. But I am unsure of what concentrations to make. I have read some articles, but they haven't mentioned...
10 February 2021 3,372 2 View
I can find several papers that give me a guide for choosing a suitable aptamer sequence 1. But how to find a property aptamer sequence 2(partially complementary to sequence 1)?
09 February 2021 3,511 1 View