I am starting out on some siRNA based knockdowns on primary cells. Would anyone recommend good services they have used in India and any advice considering the advantage of modified and fluorophore conjugated siRNAs from your experience..
Purification of gst tagged protein
25 July 2024 1,720 0 View
In the context of Electron Spin Resonance (ESR) spectroscopy, under what conditions is an absorption spectrum considered to be a single absorption spectrum?
25 July 2024 2,325 1 View
Is any specific sample preparation required to perform VSM measurement of NdFeB alloy powder to get a square-shaped M-H loop? Or is it mandatory to make magnetically aligned and sintered pellets...
09 July 2024 1,969 0 View
Technology has curbed the prowess of critical thinking by the brain. What next, after wastage of quintillions - trillions or dollars / Euros for over 100 years by gadgeteers / device-loving...
30 May 2024 9,470 0 View
Like higher dislocation density, it becomes a barrier to crack growth or becomes easy for it.
30 May 2024 7,413 2 View
"How will it affect the material? Is a higher KAM value beneficial for restricting cracks, or is a lower value more effective?"
30 May 2024 9,211 0 View
I want to know about water contact angle of silica films without using any surfactants.
29 May 2024 4,526 3 View
Primer data sheet attached
29 May 2024 1,249 2 View
need paper of IIT khargaur and energy IIT mumbai
26 May 2024 4,316 1 View
I have had the misfortune to see a quadriplegic female aged 11 years with severe spasticity ++++ following asphyxia due to laryngeal spams and cerebral anoxia growing up with her disability...
26 May 2024 6,799 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
For a partition of an integer \lambda, what does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition, , with \lambda_j being the j-th element of \lambda?
23 July 2024 8,008 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?
18 July 2024 9,831 0 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
I need Anti-Claudin8 Primary and Anti-Claudin2 conjugated Ab for my immunofluorescence experiment. If anybody has worked on it. Please share the accession number/details.
14 July 2024 485 0 View
When preparing a graph of fluorophore intensity vs. analyte concentration, the linearity shows an R² of 0.999. However, when plotting the same data as Fo/F vs. analyte concentration, the linearity...
13 July 2024 7,131 0 View