Giulio Preta

5 Questions 20 Answers 0 Followers

Questions related from Giulio Preta

Why our hepatocytes are not attaching to the plates?

We are isolating hepatocytes from mouse liver and trying to culture them in 6 or 24 well plates. The isolation is carried out according to the protocol and plating is done using William E medium...

19 April 2023 9,636 4 View

Which fluorophore for my FRAP experiments?

Hello, I have to perform FRAP experiments using fluorescent labeled cholesterol tBLM. According to lateral diffusion properties, which fluorophore is more indicated for photobleaching? We...

11 November 2015 2,220 1 View

ARe there specific DAMPs for particular form of cell death?

Toxic insults result in cell damage which can lead to apoptosis, necrosis or autophagy. DAMPs are released by damaged cells in a passive way. My question is related to the possibility that a DAMP...

13 May 2015 8,877 2 View

Is there a website which reports all the known substrates of a particular enzyme?

Does anyone know an internet site where you can check (with related references) all the known substrates for a particular enzyme?

13 May 2015 745 3 View

Is cholesterol required for membrane ruffling?

Membrane ruffling is the formation of motile cell surface protrusions containing a meshwork of newly polymerized actin filaments. Since cholesterol is essential for cellular fluidity I thought it...

12 January 2015 4,322 3 View