Can I use Hi-T4™ DNA Ligase for Gibson Assembly?

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Harmonising taxon names in biodiversity data - what are the best tools?

Hi all, I am trying to harmonise taxonomic information in a dataset from a biodiversity study. So far I've tried several functions within the taxize package in RStudio. I was referring to...

27 March 2024 10,032 2 View

Why our hepatocytes are not attaching to the plates?

We are isolating hepatocytes from mouse liver and trying to culture them in 6 or 24 well plates. The isolation is carried out according to the protocol and plating is done using William E medium...

18 April 2023 9,604 4 View

Best ATAC-Seq Pipeline?

Soon I will have to analyze some ATAC-Seq data, I was evaluating the pipelines currently available ... that it is simple to install (maybe conda) and to use ... I was...

19 January 2022 7,188 1 View

What's the highest resolution free satellite basemap I can download for use in QGIS?

So far I have found that Google Satellite downloaded via the QuickMapServices plugin in QGIS gives me the best resolution. However, if anyone could suggest anything with an even better resolution,...

02 December 2021 1,937 3 View

What could cause a low dissolved-oxygen concentration in freshwater springs?

I took a series of DO concentration readings at freshwater springs distributed over a large geographical area. Four weeks elapsed between the first measurement and the last. Each site was sampled...

21 November 2021 1,033 10 View

Recent data sources on calories of animal feed from human edible products (wheat, soy, corn, ..) supplied to livestock farmin in European countries ?

Some data are in (Stuart, 2009); more recent ones ? Thank you so much !

07 July 2021 3,079 2 View

Hourly electricity and heat demand of household dataset?

Dear all, I would kindly ask if anyone could suggest a dataset where it is collected hourly demand of electricity and heat demand of household (or commercial building) of at least one year. If...

22 May 2020 1,070 5 View

Is it possible to export the modified mesh after using the adjoint based solver on ANSYS Fluent?

After having changed the mesh using the adjoint solver optimiser on ANSYS Fluent, I am trying to export the modified mesh so that I can use it on another Fluent project. Can this be done? If not,...

12 April 2020 6,119 0 View

Is it possible to etch a material (wafer silicon) with palmsens (pstrace)?

Hello everyone! I'd like to try the electrochemical etching of a silicon wafer, but I need to work with a two-electrode configuration (a strip of aluminum foil as a back-contact and a platinum...

26 December 2019 4,700 5 View

What is the best redox potential for groundwater bioremediation of MTBE/ETBE in AEROBIC condition?

By applying a patented technology we can induce aerobic condition in the groundwater of a remediation site. Simultaneously we can influence the redox potential. I am struggling to find a...

25 July 2019 2,895 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View