What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Hye All, Greetings, How can i get surface run off water data?

this is for my thesis project, so this is the title of my thesis, the effect of land use on hydrological components in Muar, Johor, Malaysia any ideas, guidance, and suggestions all? Thank You, :)

02 February 2021 265 5 View

How can I convert netcdf file to a binary file in matlab?

Dear all, I have monthly nutrients data from WOA (in a separate NetCDF file) extracted for a specific region. I would like to convert these data directly into a bin file (one of the separate...

30 January 2021 9,241 2 View

What is the difference of FTIR for functional properties and structural properties of protein?

I need to send my sample to outsource laboratory and how can i tell them that i want to run the FTIR for structural properties of protein? is there any differenece between FTIR for functional...

15 January 2021 8,992 1 View

What's the correct way of treating ordinal variable as continuous in regression?

08 January 2021 920 16 View

Does low pHPZC (Zero point charge) indicates higher negative charged surface ?

I found that, there is a difference in pHPZC value when higher impregnation ratio of acid on adsorbent surface. Lower pHPZC value when higher ratio of acid impregnation and adsorption performance...

05 January 2021 7,224 3 View

Why do I see many vacuoles on my cancer cells?

I am currently growing BICR31 cancer cells for conditioned media collection, and as I passaged it I have seen many bubbles/vacuoles - like structures. Cells were healthy during the first split...

05 January 2021 2,320 5 View

How to design reverse primer with ambiguous nucleotides?

How to design, reverse primer with ambiguous nucleotides?

03 January 2021 6,762 4 View

Why does the DNA's elution decrease after centrifuging with spin column?

02 January 2021 987 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View