Need XRD pattern. Can anyone help?

14 September 2020 7,251 4 View

How to identify marker genes from Single Cell RNA seq data?

I am currently working with scRNA-seq data. I have to rank or score genes based on how spatially expressed they are. Is there any good measure or tool to do that?

23 August 2020 3,740 3 View

Fastq file has number code rather than nucleotide in the sequence, Why?

What's wrong with the following fastq file? Why don't I see any nucleotides? @SRR867022.1 s0205_20091123_4_Boot2_1_2_22_F3...

16 February 2020 7,784 5 View

I have time series data of 6 variables integrated with order more than 1. Should I use Johansen or E Granger test to check co-integration?

I have learned that E. Granger test is better when there are 2 variables. But in my case there are 6 of them. Johansen Test assumes that variables are either stationary or become stationary...

30 December 2017 8,856 4 View

How to get the promoter sequence of a gene? I want to get it from RSAT Plants. Do I have to get the upstream sequence form a gene or mRNA?

I want to get the promoter sequence of some genes. Should I get it from upstream of the gene or mRNA? How is gene defined in RSAT plants?

23 August 2017 5,770 4 View

What is given in gene expression csv file from BDGP?

03 December 2016 7,199 3 View

HOw to convert pdf or autocad drawing in to csv or dbd file?

I want to plot the city map on the transcad software

22 November 2016 6,897 3 View

What are the applications of Pyrene monomer emission in the solid state compared to Pyrene excimer in the solid state?

I have a material having solid state pyrene monomer emission, I want to know its advantages over its excimer emission, can anyone please guide

16 February 2015 4,707 2 View

No title

15 December 2014 4,388 15 View

Is it possible to make mutation in stopcodon at the end of an ORF by simply changing stopcodon bases in the reverse primer?

13 September 2014 5,980 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

QPCR troubleshooting - what could cause an increasing ROX signal?

Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....

08 February 2021 419 3 View