How to identify marker genes from Single Cell RNA seq data?

I am currently working with scRNA-seq data. I have to rank or score genes based on how spatially expressed they are. Is there any good measure or tool to do that?

23 August 2020 3,740 3 View

Fastq file has number code rather than nucleotide in the sequence, Why?

What's wrong with the following fastq file? Why don't I see any nucleotides? @SRR867022.1 s0205_20091123_4_Boot2_1_2_22_F3...

16 February 2020 7,784 5 View

How to get the promoter sequence of a gene? I want to get it from RSAT Plants. Do I have to get the upstream sequence form a gene or mRNA?

I want to get the promoter sequence of some genes. Should I get it from upstream of the gene or mRNA? How is gene defined in RSAT plants?

23 August 2017 5,770 4 View

What is given in gene expression csv file from BDGP?

03 December 2016 7,199 3 View

HOw to convert pdf or autocad drawing in to csv or dbd file?

I want to plot the city map on the transcad software

22 November 2016 6,897 3 View

No title

15 December 2014 4,388 15 View

Is it possible to make mutation in stopcodon at the end of an ORF by simply changing stopcodon bases in the reverse primer?

13 September 2014 5,980 6 View

How to calculate filter paper unit (FPU) activity of cellulase?

I have done FPU activity for cellulase. The enzyme is in solid form. At 0.2 mg of quantity in 1.5 ml buffer+50 mg whatman filter paper-1 it gives 2 mg glucose in 60 min. (as per NREL protocol),...

01 January 1970 2,150 9 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

No module named 'tensorflow.keras.backend.tensorflow_backend' ?

System Description Cuda 11.2 GeForce GTX 1080ti tensorflow version 2.4.1 keras version 2.2.0 python version 3.8 I was getting the below error when I try to run my code on the...

25 February 2021 6,673 2 View

Which DL architecture is better to work on handwritten data?

I am working in the area of Handwriting recognition for Bangla. As there are many DL architecture, I am confused which one to select.

15 February 2021 4,996 1 View

PV system design. 3d modelling and Analysis?

I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...

13 February 2021 8,126 1 View

Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

Schrodinger Desmond runs fine on one GPU, but crashes on other GPU?

Hi, We are having issues using Desmond on our workstation. Simulations run fine on one of our gpus ("Gpu0") but crash consistently on the other gpu ("gpu1" - which is also set up as the display...

01 February 2021 5,866 3 View

Why should we use Sustainable climate design?

Sustainable design is a set of cognitive and analytical abilities, knowledge of the environment and ample practical to incorporate things into the world of microbes, plants and animals, and the...

25 January 2021 7,816 8 View

Any good (up-to-date) CUDA programming book?

I've been looking for a decent and up-to-date book about CUDA programming, yet it has turned out to be more difficult than I expected. So far I've used CUDA by example (from Sanders & Kandrot)...

21 January 2021 7,771 4 View

What are the future research avenues in context of Urban Street, Outdoor thermal comfort and Neural Networks?

Dear academic community, I am a young researcher from architectural background. I am looking for possible novel approaches to evaluate outdoor thermal comfort in urban streets using artificial...

20 January 2021 7,964 3 View

Why the protein moved out of the solvation box after minimization using OpenMM?

Hello, I am trying to perform MD simulation for the protein-ligand system and I got this after Minimization. I used openMM for minimization and I used ambertool for protein-ligand...

20 January 2021 1,922 2 View