How to interpret the RDF plots?

I have studied the structural changes of a protein in 3 different concentrations of ionic liquids (50 mM, 500 mM, and 1M). On analysis of the RDF plots, I find that the magnitude of the RDF plot...

05 October 2020 4,972 2 View

How to design of PID controller using ANN?

27 March 2020 5,699 3 View

Which is the best way to find the number of water molecules around the protein?

Dear all, I am trying to calculate the number of water molecules at a distance of 0.35A from the protein. I used two gmx select GROMACS codes which have given me different answers. 1. gmx_mpi...

17 March 2020 9,117 3 View

Fastq file has number code rather than nucleotide in the sequence, Why?

What's wrong with the following fastq file? Why don't I see any nucleotides? @SRR867022.1 s0205_20091123_4_Boot2_1_2_22_F3...

16 February 2020 7,784 5 View

How to use neural network in MPC?

I implemented model predictive control for PMSM in Matlab. I want to incorporate neural network in my work. My system is MIMO system. Give me some suggestions.

14 January 2020 3,542 2 View

How to interpret the RDF plot?

Hi, I am studying the stabilization of proteins using different solvents using GROMACS. Attached is the RDF of the cation (black) and anion (red) around the protein. I used "gmx_mpi rdf com rdf...

18 June 2018 7,466 3 View

Can the activity of proteins be studied using molecular dynamic simulations?

I have been studying the conformational changes and stability of proteins in different solvents using MolecularDynamic Simulations. Wonder whether changes in activity of enzymes could also be...

06 April 2018 9,153 4 View

Dear friends i am need of the urban growth detection related works survey paper in satellite image ?

detect the urban growth using satellite image

08 October 2017 9,888 5 View

How to get the promoter sequence of a gene? I want to get it from RSAT Plants. Do I have to get the upstream sequence form a gene or mRNA?

I want to get the promoter sequence of some genes. Should I get it from upstream of the gene or mRNA? How is gene defined in RSAT plants?

23 August 2017 5,770 4 View

Please help with geometry optimisation in GAMESS- shift in double bond

25 May 2017 2,299 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View