How to select mobile phase of HPTLC methods validation if drug pka value id 3.5 which factor we consider salection of mobile phases?
Hello Bhushan,
For Method validation by HPTLC you have to check all Validation parameters as per ICH guidelines.
also u mentioned your drug has Pka value 3.5 .
in what solvent does it soluble?
Same parameters as identified in ICH Q2r1 (analytical method validation).
Hi,
The papers could be very useful:
Article HPTLC method development and validation: An overview
Book HPTLC Method development and Validation
Article HPTLC Method Development and Validation: Strategy to Minimiz...
Article Development and Validation of HPTLC Method for Estimation of...
Best regards
Tomasz
All parameters as per ICH Q2(R1) guideline
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