Is the negative news notification affect human brain psychologically?

life is full of the bad and sad news that may affect people life

04 May 2019 4,952 4 View

To what extent is the boiling method acceptable in PCR and biotechnology applications?

Boiling method is one of the simplest methods of DNA extraction, is it sufficient for the different applications of biotechnology

09 October 2018 705 0 View

What is contig mapping?

DNA consist of series of regions coded for the whole genome.

05 June 2018 4,777 0 View

What is the statement used to describe the process of synthesizing oligonucleotides directly on the glass slide?

There is a process used for synthesis oligonucleotides on the glass slide

05 June 2018 6,207 1 View

How is data mining useful to biotechnology research?

Data for DNA sequencing that taking from organisms need to use computers to determine the differences between these organisms.

05 June 2018 4,974 0 View

What makes a bone cell a bone cell as opposed to a liver cell lies largely in the set of genes that the cell expresses?

every somatic cell in the body has basically the same DNA.

04 May 2018 1,441 0 View

What is the role of chloroform in DNA isolation?

chloroform is one of several chemical materials used in the extraction of DNA.

03 April 2018 3,370 2 View

What is the role of Isoamyl alcohol in DNA isolation?

Isoamyl alcohol is one of several chemical materials used in the extraction of DNA.

03 April 2018 8,552 2 View

What is the role of ethanol in DNA isolation?

one of the important steps in the extraction of DNA we use ethanol.

03 April 2018 3,720 0 View

What is the importance of proteinase K in DNA extraction ?

In the process of DNA extraction, we use proteinase K.

02 March 2018 4,461 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

No title

17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View