I would like to do method validation for co-trimoxazole tablet but couldn't find the proper neutralizer to deactivate co-trimoxazole active.
Without deactivating i cant get bacteria,yeast and mould recovery.
Co-trimoxazole is a combination of trimethoprim and sulfamethoxazole.
This paper discusses the resistance mechanisms.
https://academic.oup.com/cid/article/32/11/1608/464214
One cause of resistance is mutation of the target enzymes making them insensitive to the drug, or horizontal acquisition of naturally resistant enzymes. You could use such resistant enzymes as selectable markers. Overexpression of the target enzymes would also work, probably.
Hello, the certain group of microorganisms
has cotrimixazole degradation activity such as Bacillus subtilis, Pseudomonas aeruginosa, Pseudomonas putida, Rhodococcus equi, Rhodococcus erythropolis, Rhodococcus rhodocrous, and Rhodococcus zopfii.
The most active is Rhodococcus equi.
If you want to validate cotrimoxazole tablet look for Pharmacopoeia method.
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