I want to design g RNA for knock out pathway gene to enhance secondary metabolites
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,704 1 View
Hai...could anyone suggest for me a journal with rapid publication in the field of microalgal CO2 sequestration.(review work)... a journal indexed in WOS, Scopus, SCIE with no publication...
06 May 2024 6,609 1 View
Greetings esteemed researchers! I am relatively new to the field and would greatly appreciate some guidance on a challenge I'm encountering in my research. We're currently engaged in a process...
25 March 2024 4,597 4 View
Hai everyone....I have a doubt in finding the DIC with basic laboratory procedure.. we don't have TOC analyzer in our laboratory... I can calculate Total alkalinity using titration method...
27 February 2024 2,286 1 View
Help me to find the DIC of water sample through traditional titration method...
22 February 2024 813 1 View
I am trying to understand the different phosphorylation states of SRSF1 protein in the cytoplasm vs nucleus and looking for specific antibodies for the phosphor state. As SR proteins include...
11 February 2024 7,241 3 View
Dear Researchers kindly suggest a molecular docking online free web server that does not involve protein + ligand but it should be for ligand + receptor. For example: patch dock web server. Thanks...
24 January 2024 9,381 3 View
Hai everyone.... I have a medium with added Na2CO3 for algal growth...after somedays algal cells will utilize the Carbonate for its growth...i need to find out how much carbonate ion is consumed...
11 October 2023 6,976 2 View
Procedure to find out dissolved inorganic carbon (DIC) through titration...kindly tell
11 October 2023 3,506 3 View
Are there any standards/ limits for respiratory deposition dose like this much microgram of Pm 2.5 is applicable for 1 hr exposure/ if any....I read many papers but I couldn't find it...Kindly...
12 July 2023 8,121 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,537 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,619 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 806 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,365 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 224 2 View