I want to design g RNA for knock out pathway gene to enhance secondary metabolites
Actually 316L Stainless Steel is the alloy. So how i can calculate for its equivalent weight?
14 May 2020 4,434 5 View
Is there a procedure to determine the wavelength of an industrial untreated raw effluent using spectrophotmeter
24 February 2020 1,931 3 View
I use PEG-1000 (poly ethylene glycol) in my synthesis process, but I only have PEG-400 and PEG-4000. So, what is an alternative to PEG-1000 ?
19 February 2020 2,142 3 View
I am conducting experimental simulations to study the PM 2.5 concentration.I have tried several chemistry options ranging from RADM2 MADE/SORGAM to MOZCART aerosol chemistry. The simulation...
21 October 2019 8,154 4 View
Dear frnds, From my point of view SSB is happening due to following reasons in double quantum wells. 1. Due to variational technique 2. symmetry in envelop function and...
19 October 2019 6,248 2 View
For a pure virus, HA can be considered as a way of representing their titre. even though it is cost effective and less labor intensive, it is less concerned. Kindly guide me in this matter.
04 July 2019 3,881 1 View
Mott-Wannier excitons are formed in a semiconductor material as a result of light-matter interactions. However, their delocalizing properties inside the crystal induce screening effects in their...
10 February 2019 6,737 1 View
Hi I need help for my thesis. My thesis is about teaching fraction concepts for primary pupils and I struggling to identify the appropriate constructivism learning approach.
17 June 2018 5,152 8 View
05 May 2017 178 4 View
Is there any user friendly software is available to perform interaction or docking between DNA with Isopropanol?
27 July 2016 151 7 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...
27 February 2021 5,681 3 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...
26 February 2021 8,399 5 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View