How i can calculate the equivalent weight of 316L SS?

Actually 316L Stainless Steel is the alloy. So how i can calculate for its equivalent weight?

14 May 2020 4,434 5 View

Methods for purification of DNA after digestion?

05 May 2017 178 4 View

Is there any user friendly software is available to perform interaction or docking between DNA with Isopropanol?

Is there any user friendly software is available to perform interaction or docking between DNA with Isopropanol?

27 July 2016 151 7 View

Does anyone have experience with Diaminonapthalene assay? What is the best solvent for DAN to be dissolved in?

I am trying to measure nitric oxide levels produced by alveolar epithelial cells following exposure to cytokines. I have only carried out griess assay and believe dan is much more efficient at...

12 July 2016 3,273 1 View

What is the correlation which relates the frequency with other parameters to get a stationary taylor bubble in vibrating liquid columns?

28 June 2016 6,909 1 View

What is the ideal concetration of DPPH for use on a 96 well plate for studying anti-oxidant activity?

03 March 2016 6,941 6 View

In plant mediated synthesis of nanomaterials, which factor is involved in the shape controlled synthesis of nanomaterials?

In plant mediated synthesis of nanomaterials, which factor is involved in the shape controlled synthesis of nanomaterials?

04 June 2013 4,655 1 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Is there a protocol for the insulin encapsulation at physiological pH?

Hello everyone, I am going to start my master thesis on the encapsulation of insulin. In almost all protocols we found an acidic pH is necessary. Unfortunately, pH

27 January 2021 3,692 5 View

About protocols used for glucose uptake in HepG2 cells?

Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...

25 January 2021 7,608 3 View

Why fouled membrane has more negative zeta potential and lower IP?

I modified PES membranes with nanoparticles (nanosilver, graphene, halloysite). They were used in UF for WWTP effluent treatment. After filtration, all membranes exhibited more negative zeta...

26 December 2020 5,676 3 View

Is it possible to calculate the sample size of a clinical trial with the Cohen formula when there is no similar article?

We are trying to design a clinical trial on type 2 diabetes patients. The main data that we want to assess include FBS, 2hpp, HbA1c, insulin, and HOMA-IR. Also, we will assess the lipid profile...

21 December 2020 2,454 3 View

Could anyone help with a 2-NBDG experiment on C2C12 cells?

I have been working with the C2C12 myotubes and measured their glucose uptake activity using an indirect glucose uptake assay (measuring how much glucose is left in the medium after stimulating...

23 November 2020 2,118 3 View